REEP2 Antibody (S326D-29)

Western Blot: REEP2 Antibody (S326D-29) [NBP2-59314]

Western Blot: REEP2 Antibody (S326D-29) [NBP2-59314] - Analysis of Rat Brain showing detection of ~30 kDa REEP1-2 protein using Mouse Anti-REEP1-2 Monoclonal Antibody, Clone S326D-29. Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug . Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-REEP1-2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~30 kDa. Other Band(s): ~22 kDa.

Immunocytochemistry/ Immunofluorescence: REEP2 Antibody (S326D-29) [NBP2-59314]

Immunocytochemistry/Immunofluorescence: REEP2 Antibody (S326D-29) [NBP2-59314] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-REEP1/2 Monoclonal Antibody at 1:50 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Mitochondrion, Mitochondrion Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane. Magnification: 60X.