Regucalcin Antibody (4B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00009104-M04

Novus Biologicals, part of Bio-Techne
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H00009104-M04
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Regucalcin Antibodies »

Product Specifications

Immunogen

RGN (NP_004674.1, 200 a.a. ~ 299 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQIPDGMCIDAEGKLWVACYNGGRVIRLDPVTGKRLQTVKLPVDKTTSCCFGGKNYSEMYVTCARDGMDPEGLLRQPEAGGIFKITGLGVKGIAPYSYAG

Specificity

RGN - regucalcin (senescence marker protein-30) (4B9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Regucalcin Antibody (4B9)

Western Blot: Regucalcin Antibody (4B9) [H00009104-M04]

Western Blot: Regucalcin Antibody (4B9) [H00009104-M04]

Western Blot: Regucalcin Antibody (4B9) [H00009104-M04] - Analysis of RGN expression in transfected 293T cell line by RGN monoclonal antibody (M04), clone 4B9. Lane 1: RGN transfected lysatE (33.3 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Regucalcin Antibody (4B9) [H00009104-M04]

Immunocytochemistry/ Immunofluorescence: Regucalcin Antibody (4B9) [H00009104-M04]

Immunocytochemistry/Immunofluorescence: Regucalcin Antibody (4B9) [H00009104-M04] - Analysis of monoclonal antibody to RGN on HeLa cell. Antibody concentration 15 ug/ml
ELISA: Regucalcin Antibody (4B9) [H00009104-M04]

ELISA: Regucalcin Antibody (4B9) [H00009104-M04]

ELISA: Regucalcin Antibody (4B9) [H00009104-M04] - Detection limit for recombinant GST tagged RGN is 3 ng/ml as a capture antibody.

Applications for Regucalcin Antibody (4B9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Regucalcin

The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in two transcript variants having different 5' UTRs, but encoding the same protein. [provided by RefSeq]

Alternate Names

EC 3.1.1.17, Gluconolactonase, GNL, RCsenescence marker protein-30, regucalcin (senescence marker protein-30), Senescence marker protein 30, SMP-30, SMP30regucalcin

Entrez Gene IDs

9104 (Human)

Gene Symbol

RGN

UniProt

NP_004674 (Human)

Additional Regucalcin Products

Product Documents for Regucalcin Antibody (4B9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Regucalcin Antibody (4B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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