Regucalcin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80850

Novus Biologicals, part of Bio-Techne
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NBP1-80850
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Regucalcin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALYSLFPDHHVKKYFDQVDISNGLDWSLDHKIFYYIDSLSYSVDAFDYDLQTGQISNRRSVYKLEKEEQIPDGMC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Regucalcin Antibody

Western Blot: Regucalcin Antibody [NBP1-80850]

Western Blot: Regucalcin Antibody [NBP1-80850]

Western Blot: Regucalcin Antibody [NBP1-80850] - Analysis using Anti-RGN antibody NBP1-80850 (A) shows similar pattern to independent antibody NBP1-80849 (B).
Immunocytochemistry/ Immunofluorescence: Regucalcin Antibody [NBP1-80850]

Immunocytochemistry/ Immunofluorescence: Regucalcin Antibody [NBP1-80850]

Immunocytochemistry/Immunofluorescence: Regucalcin Antibody [NBP1-80850] - Staining of human cell line Hep G2 shows localization to nucleus. Antibody staining is shown in green.
Regucalcin Antibody

Immunohistochemistry: Regucalcin Antibody [NBP1-80850] -

Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
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Applications for Regucalcin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1.0 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Regucalcin

Regucalcin is encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in two transcript variants having different 5' UTRs, but encoding the same protein.

Alternate Names

EC 3.1.1.17, Gluconolactonase, GNL, RCsenescence marker protein-30, regucalcin (senescence marker protein-30), Senescence marker protein 30, SMP-30, SMP30regucalcin

Entrez Gene IDs

9104 (Human)

Gene Symbol

RGN

Additional Regucalcin Products

Product Documents for Regucalcin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Regucalcin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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