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RelB Antibody (HL2222) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25623

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25623

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2222

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RelB. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RelB Antibody (HL2222) - Azide and BSA Free

RelB Antibody (HL2222) - Azide and BSA Free

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] -

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (NBP3-25623) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RelB Antibody (HL2222) - Azide and BSA Free

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] -

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (NBP3-25623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RelB Antibody (HL2222) - Azide and BSA Free

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] -

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (NBP3-25623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for RelB Antibody (HL2222) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RelB

RelB, also known as I-Rel, is a member of the Rel transcription factor family which also include; RelA-p65, c-Rel, NF-kB1 p100/52 and NF-kB2 p105/50 (1). Unlike other Rel-related protein, RelB does not bind to DNA or to inhibitor IkB, but is instead sequestered in the cytoplasm by precursor p100. Once p100 is processed, the p52/RelB complex translocates into the nucleus (2). RelB is also the transcription factor that has been associated most directly with dendritic cells (DCs) differentiation and function (3). It is upregulated and translocated into the nuclei of DC in response to various stimuli. RelB is also essential for generating a specific DC subset (CD11c+/CD11b+/CD8-/Dec205-) from hematopoietic stem cells (5).

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog B

Alternate Names

IREL

Gene Symbol

RELB

Additional RelB Products

Product Documents for RelB Antibody (HL2222) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RelB Antibody (HL2222) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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