Renilla Luciferase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42914

Novus Biologicals, part of Bio-Techne
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NBP2-42914
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Key Product Details

Species Reactivity

Renilla reniformis

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Renilla Luciferase Antibodies »

Product Specifications

Immunogen

Full length Renilla Luciferase recombinant protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Renilla Luciferase Antibody

Western Blot: Renilla Luciferase Antibody [NBP2-42914]

Western Blot: Renilla Luciferase Antibody [NBP2-42914]

Western Blot: Renilla Luciferase Antibody [NBP2-42914] - Renilla Luciferase antibody detects Renilla Luciferase protein by Western blot analysis. Non-transfected (-) and Renilla Luciferase -transfected (+, ) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Renilla Luciferase antibody at a dilution of 1:2000.

Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Renilla Luciferase stained by Renilla Luciferase antibody diluted at 1:500.Blue: Fluoroshield with DAPI.

Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Renilla Luciferase stained by Renilla Luciferase antibody diluted at 1:500.Blue: Fluoroshield with DAPI.

Applications for Renilla Luciferase Antibody

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Western Blot

1:500-1:3000
Application Notes
IHC-Wm, ICC/IF Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Renilla Luciferase

Luciferase is a generic term for a group of oxidative enzymes used in bioluminescence. Firefly (Photinus pyralis) and bacterial luciferase enzymes are commonly used in assay systems such as cell viability assays, reporter gene assays, and for in vivo imaging. Bacterial luciferases are flavoenzymes composed of two subunits each encoded by the luxA and luxB genes, while the firefly luciferase is a single polypeptide specified by the luc gene (1). Firefly luciferase (theoretical molecular weight: 61 kDa) oxidizes the substrate luciferin to oxyluciferin in a bioluminescent reaction requiring Mg2+ and ATP (2,3). This reaction produces a flash of yellow-green light with an emission peak around 560nm that can be detected by a luminometer (3). Firefly luciferase has become one of the more widely used reporter proteins and is an excellent tool for the study of gene expression, given that the amount of light emitted is directly proportional to luciferase activity (4).

The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.

References

1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9

2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005

3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b

4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036

Alternate Names

Green-shifted Renilla luciferase;Renilla-luciferin 2-monooxygenase;Renilla-type luciferase

Additional Renilla Luciferase Products

Product Documents for Renilla Luciferase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Renilla Luciferase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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