Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2F10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Renin Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 131-406 of human REN(NP_000528) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Renin Antibody (OTI2F10) - Azide and BSA Free

Western Blot: Renin Antibody (OTI2F10)Azide and BSA Free [NBP2-73851]

Western Blot: Renin Antibody (OTI2F10)Azide and BSA Free [NBP2-73851]

Western Blot: Renin Antibody (OTI2F10) - Azide and BSA Free [NBP2-73851] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Renin.
Immunohistochemistry: Renin Antibody (OTI2F10) - Azide and BSA Free [NBP2-73851]

Immunohistochemistry: Renin Antibody (OTI2F10) - Azide and BSA Free [NBP2-73851]

Immunohistochemistry: Renin Antibody (OTI2F10) - Azide and BSA Free [NBP2-73851] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Renin Antibody (OTI2F10) - Azide and BSA Free [NBP2-73851]

Immunohistochemistry: Renin Antibody (OTI2F10) - Azide and BSA Free [NBP2-73851]

Immunohistochemistry: Renin Antibody (OTI2F10) - Azide and BSA Free [NBP2-73851] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for Renin Antibody (OTI2F10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Renin

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia.

Alternate Names

Angiotensinogenase, REN

Gene Symbol

REN

Additional Renin Products

Product Documents for Renin Antibody (OTI2F10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Renin Antibody (OTI2F10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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