Renin R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1318

Novus Biologicals, part of Bio-Techne
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NB100-1318
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Renin R Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SIIYRMTNQKIRMD corresponding to C-Terminus according to NP_005756.2.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22911790).

Localization

Potential integral membrane protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Renin R Antibody

Western Blot: Renin R Antibody [NB100-1318]

Western Blot: Renin R Antibody [NB100-1318]

Western Blot: Renin R Antibody [NB100-1318] - (0.5ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry: Renin R Antibody [NB100-1318]

Immunohistochemistry: Renin R Antibody [NB100-1318]

Immunohistochemistry: Renin R Antibody [NB100-1318] - Immunofluorescence staining of LV anterior wall at day 3 after gene transfer showing Renin R predominantly in cardiomyocytes.A) DAPI (blue),B) prolyl 4-hydroxylase staining for fibroblasts (red),C) Renin R (green) andD, Merge. Representative image is shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22911790/) licensed under a CC-BY license.
Flow Cytometry: Renin R Antibody [NB100-1318]

Flow Cytometry: Renin R Antibody [NB100-1318]

Flow Cytometry: Renin R Antibody [NB100-1318] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for Renin R Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.3 - 1.0 ug/ml
Application Notes
Western blot: Approx 38-39kDa band observed in Human Cerebellum and in Human and Rat Heart lysates (calculated MW of 39.0kDa according to Human NP_005756.2 and Rat NP_001007092.1). Primary incubation 1 hour at room temperature.
Use in ICC/IF reported in scientific literature (PMID: 34228176).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Renin R

This gene encodes a protein which is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.

Long Name

Renin Receptor

Alternate Names

ATP6AP2, ATP6IP2, ATP6M8-9, CAPER, ELDF10, HT028, M8-9, MRXE, MSTP009, N14F, ReninR, XMRE

Entrez Gene IDs

10159 (Human); 70495 (Mouse)

Gene Symbol

ATP6AP2

UniProt

O75787

Additional Renin R Products

Product Documents for Renin R Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Renin R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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