RENT1/UPF1/hUPF1 Antibody (5D5C4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16544

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16544-100ul
NBP3-16544-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5D5C4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RENT1/UPF1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RENT1/UPF1/hUPF1 (Q92900). MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGAAAGQLDAQVGPEGILQNGAVDDSVAKTSQLL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RENT1/UPF1/hUPF1 Antibody (5D5C4)

Western Blot: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544]

Western Blot: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544]

Western Blot: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544] - Western blot analysis of extracts of various cell lines, using RENT1/UPF1/hUPF1 Rabbit mAb (NBP3-16544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544]

Immunocytochemistry/ Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544]

Immunocytochemistry/Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544] - Immunofluorescence analysis of C6 cells using RENT1/UPF1/hUPF1 Rabbit mAb (NBP3-16544) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544]

Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544]

Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RENT1/UPF1/hUPF1 antibody (NBP3-16544). Western blot was performed from the immunoprecipitate using RENT1/UPF1/hUPF1 antibody (NBP3-16544) at a dilition of 1:1000.

Applications for RENT1/UPF1/hUPF1 Antibody (5D5C4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RENT1/UPF1

hUPF1 encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq]

Long Name

Regulator of Nonsense Transcripts 1

Alternate Names

NORF1, UPF1

Gene Symbol

UPF1

Additional RENT1/UPF1 Products

Product Documents for RENT1/UPF1/hUPF1 Antibody (5D5C4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RENT1/UPF1/hUPF1 Antibody (5D5C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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