RENT1/UPF1/hUPF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89641

Novus Biologicals, part of Bio-Techne
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NBP1-89641
NBP1-89641-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RENT1/UPF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RENT1/UPF1/hUPF1 Antibody

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-89641]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-89641]

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-89641] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: RENT1/UPF1/hUPF1 Antibody [NBP1-89641]

Immunocytochemistry/ Immunofluorescence: RENT1/UPF1/hUPF1 Antibody [NBP1-89641]

Immunocytochemistry/Immunofluorescence: RENT1/UPF1/hUPF1 Antibody [NBP1-89641] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RENT1/UPF1/hUPF1 Antibody [NBP1-89641]

Immunohistochemistry-Paraffin: RENT1/UPF1/hUPF1 Antibody [NBP1-89641]

Immunohistochemistry-Paraffin: RENT1/UPF1/hUPF1 Antibody [NBP1-89641] - Staining of human duodenum shows strong granular positivity in glandular cells.
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Applications for RENT1/UPF1/hUPF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RENT1/UPF1

hUPF1 encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq]

Long Name

Regulator of Nonsense Transcripts 1

Alternate Names

NORF1, UPF1

Gene Symbol

UPF1

Additional RENT1/UPF1 Products

Product Documents for RENT1/UPF1/hUPF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RENT1/UPF1/hUPF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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