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Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25625

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25625

Key Product Details

Species Reactivity

Validated:

Virus

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1245

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary

Immunogen

Recombinant protein encompassing a sequence within the C-term region of Respiratory Syncytial Virus Nucleoprotein. The exact sequence is proprietary.

Reactivity Notes

Respiratory Syncytial Virus

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images

Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free

Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free [NBP3-25625] -

Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free [NBP3-25625] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1245] (NBP3-25625) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free

Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free [NBP3-25625] -

Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free [NBP3-25625] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Respiratory Syncytial virus Nucleoprotein antibody [HL1245] (NBP3-25625) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free

ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free [NBP3-25625] -

ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1245) - Azide and BSA Free [NBP3-25625] - Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below. Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1245] (NBP3-25625) (5 ug/mL) Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1291] (NBP3-25629) (1 ug/mL) Please notice that NBP3-25629 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25625. Please contact us for custom HRP-conjugated antibody.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Respiratory Syncytial Virus Nucleoprotein

Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.

Additional Respiratory Syncytial Virus Nucleoprotein Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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