Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25632

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25632
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Key Product Details

Species Reactivity

Validated:

Virus

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1297

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Respiratory Syncytial Virus Nucleoprotein Antibodies »

Product Summary

Immunogen

Recombinant protein encompassing a sequence within the center region of Respiratory Syncytial Virus Nucleoprotein. The exact sequence is proprietary.

Reactivity Notes

Respiratory Syncytial Virus type A

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images

Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free

ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free [NBP3-25632] -

ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free [NBP3-25632] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. The amount of capture and detection antibodies are 5 and 1 ug/mL, respectively. Pair 1: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (NBP3-25626) Detection: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) conjugated with HRP Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (NBP3-25626) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) conjugated with HRP Pair 3: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (NBP3-25626) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630) conjugated with HRP Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.
Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free

ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free [NBP3-25632] -

ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free [NBP3-25632] - Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag using ELISA pairs below. Pair 1: Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP (1 ug/mL) Pair 2: Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) (5 ug/mL) Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (NBP3-25627) conjugated with HRP (1 ug/mL) Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one. Please contact us for custom HRP-conjugated antibody.
Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free

ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free [NBP3-25632] -

ELISA: Respiratory Syncytial Virus Nucleoprotein Antibody (HL1297) - Azide and BSA Free [NBP3-25632] - Indirect ELISA analysis performed by coating plate with recombinant HEK293 expressed, N-terminal respiratory syncytial virus type A nucleoprotein (50 ng). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (160000-1.9*10^-2 pM) below. Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody. Antibodies: 1. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (NBP3-25632) 2. Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (NBP3-25479) 3. Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (NBP3-25630)
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Applications

Application
Recommended Usage

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Respiratory Syncytial Virus Nucleoprotein

Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.

Additional Respiratory Syncytial Virus Nucleoprotein Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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