Retinol Binding Protein RBP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33862

Novus Biologicals, part of Bio-Techne
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NBP2-33862
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Retinol Binding Protein RBP Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MLVNENFEEYLRALDVNVALRKIANLLKPDKEIVQDGDHMIIRTLSTFRNYIMDFQVGKEFEEDLTGIDDRKCMTTVSWDGDKLQCVQKGEKEGRGWTQWIEGDELHLEMRVEGVVCKQVFKKVQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Retinol Binding Protein RBP Antibody

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-33862]

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-33862]

Western Blot: Retinol Binding Protein RBP Antibody [NBP2-33862] - Analysis in control (vector only transfected HEK293T lysate) and RBP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: Retinol Binding Protein RBP Antibody [NBP2-33862]

Immunocytochemistry/ Immunofluorescence: Retinol Binding Protein RBP Antibody [NBP2-33862]

Immunocytochemistry/Immunofluorescence: Retinol Binding Protein RBP Antibody [NBP2-33862] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody [NBP2-33862]

Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody [NBP2-33862]

Immunohistochemistry-Paraffin: Retinol Binding Protein RBP Antibody [NBP2-33862] - Staining in human fallopian tube and prostate tissues using anti-RBP1 antibody. Corresponding RBP1 RNA-seq data are presented for the same tissues.
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Applications for Retinol Binding Protein RBP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1:100 - 1:250
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Retinol Binding Protein RBP

RBP1 is the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. The gene harbors four exons encoding 24, 59, 33, and 16 amino acid residues respectively. The second intervening sequence alone occupies 19 kb of the 21 kb of the gene.

Alternate Names

C, Cellular retinol-binding protein, CRABP-I, CRBP1CRBP-I, CRBPCellular retinol-binding protein I, CRBPI, RBPC, retinol binding protein 1, cellular, retinol-binding protein 1, retinol-binding protein 1, cellular

Gene Symbol

RBP1

Additional Retinol Binding Protein RBP Products

Product Documents for Retinol Binding Protein RBP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Retinol Binding Protein RBP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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