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Retinol Saturase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58103

Novus Biologicals, part of Bio-Techne
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NBP2-58103
NBP2-58103-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Retinol Saturase Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SSPNPFSEDVKRPPAPLVTDKEARKKVLKQAFSANQVPEKLDVVVIGSGFGGLAAAAILAKAGKRVLVLEQHTKAGGCCHTFGKNGLEFDTGIHYIGRMEEGSIGRFILDQITEGQLDWAPLSSPFDIMVLEGPNGRKEYPM

Reactivity Notes

Mouse 88%, Rat 89%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Retinol Saturase Antibody

Western Blot: Retinol Saturase Antibody [NBP2-58103]

Western Blot: Retinol Saturase Antibody [NBP2-58103]

Western Blot: Retinol Saturase Antibody [NBP2-58103] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: Retinol Saturase Antibody [NBP2-58103]

Immunocytochemistry/ Immunofluorescence: Retinol Saturase Antibody [NBP2-58103]

Immunocytochemistry/Immunofluorescence: Retinol Saturase Antibody [NBP2-58103] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.

Applications for Retinol Saturase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Retinol Saturase

Retinol saturase carrying out the saturation of the 13-14 double bond of all-trans-retinol to produce all-trans-13,14-dihydroretinol. Has activity toward all-trans-retinol as substrate. Does not use all-trans-retinoic acid nor 9-cis, 11-cis or 13-cis-retinol isomers as substrates. May play a role in the metabolism of vitamin A (By similarity)

Alternate Names

All-trans-13,14-dihydroretinol saturase, all-trans-retinol 13,14-reductase, EC 1.3.99.23, FLJ20296, retinol saturase (all-trans-retinol 13,14-reductase), RetSat

Gene Symbol

RETSAT

Additional Retinol Saturase Products

Product Documents for Retinol Saturase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Retinol Saturase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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