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RFC3 Antibody (1C6)

Novus Biologicals, part of Bio-Techne | Catalog # H00005983-M01

Novus Biologicals, part of Bio-Techne
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H00005983-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RFC3 (AAH00149, 257 a.a. ~ 356 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETANAIVSQQTPQRLLEVRGRLYELLTHCIPPEIIMKGLLSELLHNCDGQLKGEVAQMAAYYEHRLQLGSKAIYHLEAFVAKFMALYKKFMEDGLEGMMF

Specificity

RFC3 (1C6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RFC3 Antibody (1C6)

Immunoprecipitation: RFC3 Antibody (1C6) [H00005983-M01]

Immunoprecipitation: RFC3 Antibody (1C6) [H00005983-M01]

Immunoprecipitation: RFC3 Antibody (1C6) [H00005983-M01] - Analysis of RFC3 transfected lysate using anti-RFC3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RFC3 monoclonal antibody.
Sandwich ELISA: RFC3 Antibody (1C6) [H00005983-M01] - Detection limit for recombinant GST tagged RFC3 is approximately 10ng/ml as a capture antibody.

Applications for RFC3 Antibody (1C6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RFC3

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, MGC5276, replication factor C (activator 1) 3 (38kD), replication factor C (activator 1) 3, 38kDa, Replication factor C 38 kDa subunit, replication factor C subunit 3, RFC, 38 kD subunit, RFC38RF-C 38 kDa subunit

Entrez Gene IDs

5983 (Human)

Gene Symbol

RFC3

OMIM

600405 (Human)

Additional RFC3 Products

Product Documents for RFC3 Antibody (1C6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RFC3 Antibody (1C6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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