RFC3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82668

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-82668
NBP1-82668-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RFC3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSTVCKKEGLNLPSQLAHRLAEKSCRNLRKALLMCEACRVQQYPFTADQEIPETDWEVYLRETANAIVSQQTPQRLLEVRGRLYELLTHCIPPEIIMKGLLSELLHNCDGQLKGEVAQMAAYYEHRLQLGSKAIYHLEAFVA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFC3 Antibody

Western Blot: RFC3 Antibody [NBP1-82668]

Western Blot: RFC3 Antibody [NBP1-82668]

Western Blot: RFC3 Antibody [NBP1-82668] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-473
Immunocytochemistry/ Immunofluorescence: RFC3 Antibody [NBP1-82668]

Immunocytochemistry/ Immunofluorescence: RFC3 Antibody [NBP1-82668]

Immunocytochemistry/Immunofluorescence: RFC3 Antibody [NBP1-82668] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: RFC3 Antibody [NBP1-82668]

Immunohistochemistry-Paraffin: RFC3 Antibody [NBP1-82668]

Immunohistochemistry-Paraffin: RFC3 Antibody [NBP1-82668] - Staining of human stomach shows strong nuclear positivity in glandular cells.

Applications for RFC3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RFC3

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, MGC5276, replication factor C (activator 1) 3 (38kD), replication factor C (activator 1) 3, 38kDa, Replication factor C 38 kDa subunit, replication factor C subunit 3, RFC, 38 kD subunit, RFC38RF-C 38 kDa subunit

Gene Symbol

RFC3

Additional RFC3 Products

Product Documents for RFC3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RFC3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.