RFC3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20134

Novus Biologicals, part of Bio-Techne
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NBP2-20134
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RFC3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RFC3. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RFC3 Antibody

Western Blot: RFC3 Antibody [NBP2-20134]

Western Blot: RFC3 Antibody [NBP2-20134]

Western Blot: RFC3 Antibody [NBP2-20134] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RFC3 Antibody [NBP2-20134]

Immunocytochemistry/ Immunofluorescence: RFC3 Antibody [NBP2-20134]

Immunocytochemistry/Immunofluorescence: RFC3 Antibody [NBP2-20134] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: RFC3 Antibody [NBP2-20134]

Immunohistochemistry-Paraffin: RFC3 Antibody [NBP2-20134]

Immunohistochemistry-Paraffin: RFC3 Antibody [NBP2-20134] - Human gastric cancer, using RFC3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for RFC3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RFC3

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Alternate Names

A1 38 kDa subunit, Activator 1 38 kDa subunit, Activator 1 subunit 3, MGC5276, replication factor C (activator 1) 3 (38kD), replication factor C (activator 1) 3, 38kDa, Replication factor C 38 kDa subunit, replication factor C subunit 3, RFC, 38 kD subunit, RFC38RF-C 38 kDa subunit

Gene Symbol

RFC3

UniProt

P40938 (Human)

Additional RFC3 Products

Product Documents for RFC3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RFC3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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