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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A 23 amino acid synthetic peptide, which represented a portion of the human Replication Factor C 4, 37 kDa subunit encoded within exon 2 (locusLink ID 5984).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFC4 Antibody

Western Blot: RFC4 Antibody [NB100-403]

Western Blot: RFC4 Antibody [NB100-403]

Western Blot: RFC4 Antibody [NB100-403] - Sample: Nuclear extract (20 ug for WB; 300 ug for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-RFC4 used at 0.5 ug/ml for WB and 2 ug/300 ug lysate for IP. Detection: Chemiluminescence with 10 sec. exposure.

Applications for RFC4 Antibody

Application
Recommended Usage

Western Blot

1:1000-1:10000
Application Notes
NB 100-403 may be used for Western Blot and immunoprecipitation. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RFC4

Human replication factor C (RFC) is a hetero-pentameric protein. RFC p37 (RFC4) encodes subunit 4 of the RFC complex, which functions in DNA checkpoint control. It plays a role in mitotic cohesion and condensation.

Alternate Names

A1, A1 37 kDa subunit, Activator 1 37 kDa subunit, Activator 1 subunit 4, MGC27291, replication factor C (activator 1) 4, 37kDa, Replication factor C 37 kDa subunit, replication factor C subunit 4, RFC 37 kDa subunit, RF-C 37 kDa subunit, RFC37replication factor C (activator 1) 4 (37kD)

Entrez Gene IDs

5984 (Human)

Gene Symbol

RFC4

Additional RFC4 Products

Product Documents for RFC4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RFC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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