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RFWD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68207

Novus Biologicals, part of Bio-Techne
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NB100-68207

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFWD3 Antibody

Immunoprecipitation: RFWD3 Antibody [NB100-68207] -

Detection of Human RFWD3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RFWD3 antibody NB100-68207 used for IP at 3 mcg/mg lysate. RFWD3 was also immunoprecipitated by rabbit anti-RFWD3 antibody NB100-68206, which recognizes an uptream epitope. For blotting immunoprecipitated RFWD3, NB100-68208 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for RFWD3 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500
Application Notes
This antibody is not recommended for Western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RFWD3

RFWD3 (ring finger and WD repeat domain 3) has been identified as a protein that is phosphorylated in response to DNA damage. The exact function of RFWD3 (ring finger and WD repeat domain 3) has not been characterized. Structurally, RFWD3 possesses a RING-type zinc finger and 3 WD repeats.

Alternate Names

EC 6.3.2.-, FLJ10520, ring finger and WD repeat domain 3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201, RNF201E3 ubiquitin-protein ligase RFWD3

Entrez Gene IDs

55159 (Human)

Gene Symbol

RFWD3

Additional RFWD3 Products

Product Documents for RFWD3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RFWD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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