RFWD3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-68208
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Predicted:
Monkey (100%), Porcine (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation (Negative), Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 724 and 774 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159).
Reactivity Notes
Orangutan, Monkey, Gorilla and Chimpanzee (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RFWD3 Antibody
![Western Blot: RFWD3 Antibody [NB100-68208]](https://resources.bio-techne.com/images/products/RFWD3-Antibody-Western-Blot-NB100-68208-img0004.jpg "Western Blot: RFWD3 Antibody [NB100-68208]")
Western Blot: RFWD3 Antibody [NB100-68208]
Western Blot: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFWD3 antibody NB100-68208 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: RFWD3 Antibody [NB100-68208]](https://resources.bio-techne.com/images/products/RFWD3-Antibody-Immunoprecipitation-NB100-68208-img0005.jpg "Immunoprecipitation: RFWD3 Antibody [NB100-68208]")
Immunoprecipitation: RFWD3 Antibody [NB100-68208]
Immunoprecipitation: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: RFWD3 was immunoprecipitated by rabbit anti-RFWD3 antibodies NB100-68206 and NB100-68207. For blotting immunoprecipitated RFWD3, NB100-68208 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for RFWD3 Antibody
Application
Recommended Usage
Western Blot
1:2000-1:10000
Application Notes
Immunoprecipitation: Not recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RFWD3
Alternate Names
EC 6.3.2.-, FLJ10520, ring finger and WD repeat domain 3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201, RNF201E3 ubiquitin-protein ligase RFWD3
Entrez Gene IDs
55159 (Human)
Gene Symbol
RFWD3
UniProt
Additional RFWD3 Products
Product Documents for RFWD3 Antibody
Product Specific Notices for RFWD3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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