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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1. The immunogen is located within the last 50 amino acids of RFX1. Amino Acid Squence: AGGESPALGPETLEPPAK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse: (89%)

Specificity

RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RFX1 Antibody - BSA Free

Western Blot: RFX1 AntibodyBSA Free [NBP2-82031]

Western Blot: RFX1 AntibodyBSA Free [NBP2-82031]

Western Blot: RFX1 Antibody [NBP2-82031] - Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 ug/ml.
RFX1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RFX1 Antibody - BSA Free [NBP2-82031] -

Immunocytochemistry/ Immunofluorescence: RFX1 Antibody - BSA Free [NBP2-82031] - Immunocytochemistry of RFX1 in Hela cells with RFX1 antibody at 5 ug/mL.
RFX1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RFX1 Antibody - BSA Free [NBP2-82031] -

Immunocytochemistry/ Immunofluorescence: RFX1 Antibody - BSA Free [NBP2-82031] - Immunofluorescence of RFX1 in Hela cells with RFX1 antibody at 20 ug/mL.

Green: RFX1 Antibody
Blue: DAPI staining

Applications for RFX1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RFX1

RFX1, also known as MHC class II regulatory factor RFX1, is a 105 kDa 979 amino acid protein found in the nucleus. The gene plays a significant role in the gene expression of MHC class II. Current research on RFX1 is being conducted in relation to hepatitis, leukemia, neuroblastoma , sickle cell anemia and adenocarcinoma. RFX1 has been linked to the methlation, immune response, spermatogenesis and localization pathways where it interacts with TADA3, SMAD1, HDAC1, ADD1 and SMAD9.

Alternate Names

EFC, EF-C, Enhancer factor C, MHC class II regulatory factor RFX, MHC class II regulatory factor RFX1, Regulatory factor X 1, regulatory factor X, 1 (influences HLA class II expression), RFX, trans-acting regulatory factor 1, Transcription factor RFX1

Gene Symbol

RFX1

Additional RFX1 Products

Product Documents for RFX1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RFX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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