RFXANK Antibody (4G10)

Novus Biologicals, part of Bio-Techne | Catalog # H00008625-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RFXANK Antibodies »

Product Specifications

Immunogen

RFXANK (NP_003712.1, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MELTQPAEDLIQTQQTPASELGDPEDPGEEAADGSDTVVLSLFPCTPEPVNPEPDASVSSPQAGSSLKHSTTLTNRQRGNEVSALPATLD

Specificity

RFXANK - regulatory factor X-associated ankyrin-containing protein (4G10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RFXANK Antibody (4G10)

Western Blot: RFXANK Antibody (4G10) [H00008625-M01]

Western Blot: RFXANK Antibody (4G10) [H00008625-M01]

Western Blot: RFXANK Antibody (4G10) [H00008625-M01] - Analysis of RFXANK expression in transfected 293T cell line by RFXANK monoclonal antibody (M01), clone 4G10.Lane 1: RFXANK transfected lysate (Predicted MW: 25.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RFXANK Antibody (4G10) [H00008625-M01]

Immunocytochemistry/ Immunofluorescence: RFXANK Antibody (4G10) [H00008625-M01]

Immunocytochemistry/Immunofluorescence: RFXANK Antibody (4G10) [H00008625-M01] - Analysis of monoclonal antibody to RFXANK on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: RFXANK Antibody (4G10) [H00008625-M01] - Detection limit for recombinant GST tagged RFXANK is approximately 3ng/ml as a capture antibody.

Applications for RFXANK Antibody (4G10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RFXANK

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Two transcript variants encoding different isoforms have been described for this gene, with only one isoform showing activation activity. [provided by RefSeq]

Alternate Names

ANKRA1Regulatory factor X subunit B, ankyrin repeat-containing regulatory factor X-associated protein, BLS, DNA-binding protein RFXANK, F14150_1, MGC138628, regulatory factor X-associated ankyrin-containing proteinAnkyrin repeat family A protein 1, RFXB, RFX-BRFX-Bdelta4

Entrez Gene IDs

8625 (Human)

Gene Symbol

RFXANK

UniProt

NP_003712 (Human)

Additional RFXANK Products

Product Documents for RFXANK Antibody (4G10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RFXANK Antibody (4G10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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