RGNEF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90880

Novus Biologicals, part of Bio-Techne
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NBP1-90880
NBP1-90880-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RGNEF Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RGNEF Antibody

Immunohistochemistry-Paraffin: RGNEF Antibody [NBP1-90880]

Immunohistochemistry-Paraffin: RGNEF Antibody [NBP1-90880]

Immunohistochemistry-Paraffin: RGNEF Antibody [NBP1-90880] - Staining in human kidney and skeletal muscle tissues using anti-ARHGEF28 antibody. Corresponding ARHGEF28 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: RGNEF Antibody [NBP1-90880]

Immunocytochemistry/ Immunofluorescence: RGNEF Antibody [NBP1-90880]

Immunocytochemistry/Immunofluorescence: RGNEF Antibody [NBP1-90880] - Staining of human cell line U-2 OS shows localization to cytosol & microtubules.
Immunohistochemistry-Paraffin: RGNEF Antibody [NBP1-90880]

Immunohistochemistry-Paraffin: RGNEF Antibody [NBP1-90880]

Immunohistochemistry-Paraffin: RGNEF Antibody [NBP1-90880] - Staining of human kidney shows high expression.
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Applications for RGNEF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RGNEF

Alternate Names

190 kDa guanine nucleotide exchange factor, p190RHOGEF, Rho-guanine nucleotide exchange factor

Entrez Gene IDs

64283 (Human)

Gene Symbol

ARHGEF28

UniProt

Q8N1W1 (Human)

Additional RGNEF Products

Product Documents for RGNEF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RGNEF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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