Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RGNEF Antibodies »

Product Summary for RGNEF Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1655 and 1705 of human 190 kDa, Rho-Guanine Nucleotide Exchange Factor using the numbering given in entry NP_001171164.1 (GeneID 64283).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RGNEF Antibody

Western Blot: RGNEF Antibody [NBP2-22282]

Western Blot: RGNEF Antibody [NBP2-22282]

Western Blot: RGNEF Antibody [NBP2-22282] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22282 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RGNEF Antibody [NBP2-22282]

Immunoprecipitation: RGNEF Antibody [NBP2-22282]

Immunoprecipitation: RGNEF Antibody [NBP2-22282] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RGNEF antibody NBP2-22282 used for IP at 6 ug/mg lysate. RGNEF was also immunoprecipitated by rabbit anti-RGNEF antibody. For blotting immunoprecipitated RGNEF, NBP2-22282 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for RGNEF Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RGNEF

Alternate Names

190 kDa guanine nucleotide exchange factor, p190RHOGEF, Rho-guanine nucleotide exchange factor

Gene Symbol

ARHGEF28

UniProt

NP_001171164.1 (Human)

Additional RGNEF Products

Product Documents for RGNEF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RGNEF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.