RGS16 Antibody (OTI4E5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01584
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4E5
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human RGS16(NP_002919) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
22.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RGS16 Antibody (OTI4E5)
Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584]
Western Blot: RGS16 Antibody (OTI4E5) [NBP2-01584] - Inverse Arg2-RGS16 regulatory relationship is identified by unbiased transcriptomics. RGS16 gene expression and protein abundance in HFrD-fed (f) AAV8-control and AAV8-Arg2 mouse livers. For box plots, the midline represents the median, boxes represent the interquartile range and whiskers show the full range of values. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001; n.s., not significant; relative to control treatment, by two-tailed Student's t-test Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-09642-8), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: RGS16 Antibody (OTI4E5) [NBP2-01584]
Immunocytochemistry/Immunofluorescence: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RGS16.Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584]
Immunohistochemistry-Paraffin: RGS16 Antibody (OTI4E5) [NBP2-01584] - Staining of paraffin-embedded Human pancreas tissue using anti-RGS16 mouse monoclonal antibody.Applications for RGS16 Antibody (OTI4E5)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RGS16
Alternate Names
A28-RGS14, A28-RGS14P, hRGS-r, regulator of G-protein signaling 16, regulator of G-protein signalling 16, Retinally abundant regulator of G-protein signaling, Retinal-specific RGS, RGSR, RGS-R
Entrez Gene IDs
6004 (Human)
Gene Symbol
RGS16
Additional RGS16 Products
Product Documents for RGS16 Antibody (OTI4E5)
Product Specific Notices for RGS16 Antibody (OTI4E5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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