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RGS2 Antibody (4C4)

Novus Biologicals, part of Bio-Techne | Catalog # H00005997-M01

Novus Biologicals, part of Bio-Techne
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H00005997-M01

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RGS2 (AAH07049, 111 a.a. ~ 211 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT

Specificity

RGS2 - regulator of G-protein signalling 2, 24kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RGS2 Antibody (4C4)

Knockdown Validated: RGS2 Antibody (4C4) [H00005997-M01]

Knockdown Validated: RGS2 Antibody (4C4) [H00005997-M01]

Knockdown Validated: RGS2 Antibody (4C4) [H00005997-M01] - Knockdown efficiencies of target proteins in HEK293 cells measured by Reverse Phase Protein Array (RPPA) and Western blotting (WB). Level of expression by WB and RPPA of targeted proteins in HEK293 cells transfected with siRNA targeting proteins of interest or non-silencing siRNA control (siCTR). Specific bands for WB and spots corresponding to the dilution of the dynamic range in RPPA are presented. Image collected and cropped by CiteAb from the following publication (https://rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.
Western Blot: RGS2 Antibody (4C4) [H00005997-M01]

Western Blot: RGS2 Antibody (4C4) [H00005997-M01]

Western Blot: RGS2 Antibody (4C4) [H00005997-M01] - Specificity of antibodies. HEK293 cells lysates were analysed by Western blotting to test the specificity of antibodies. Image collected and cropped by CiteAb from the following publication (https://rbej.biomedcentral.com/articles/10.1186/1477-7827-11-100) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: RGS2 Antibody (4C4) [H00005997-M01]

Immunohistochemistry-Paraffin: RGS2 Antibody (4C4) [H00005997-M01]

Immunohistochemistry-Paraffin: RGS2 Antibody (4C4) [H00005997-M01] - Analysis of monoclonal antibody to RGS2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Applications for RGS2 Antibody (4C4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RGS2

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 2 belongs to this family. The protein acts as a mediator of myeloid differentiation and may play a role in leukemogenesis. (provided by RefSeq)

Alternate Names

Cell growth-inhibiting gene 31 protein, G0 to G1 switch regulatory 8, 24kD, G0/G1 switch regulatory protein 8, G0S8cell growth-inhibiting protein 31, regulator of G-protein signaling 2, regulator of G-protein signaling 2, 24kDa, regulator of G-protein signalling 2, 24kD, regulator of G-protein signalling 2, 24kDa

Entrez Gene IDs

5997 (Human)

Gene Symbol

RGS2

OMIM

600861 (Human)

Additional RGS2 Products

Product Documents for RGS2 Antibody (4C4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RGS2 Antibody (4C4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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