Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all RHBDD1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human RHBDD1. The immunogen is located within the last 50 amino acids of RHBDD1. Amino Acid Squence: WDRGNTRNSPPPYG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RHBDD1 Antibody - BSA Free

RHBDD1 Antibody - BSA Free

Western Blot: RHBDD1 Antibody - BSA Free [NBP1-76248] -

Western Blot: RHBDD1 Antibody - BSA Free [NBP1-76248] - WB Validation in Mouse Heart.Loading: 15 ug of lysate Antibodies: RHBDD1, 1 ug/ml, 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 5 min
RHBDD1 Antibody - BSA Free

Western Blot: RHBDD1 Antibody - BSA Free [NBP1-76248] -

Western Blot: RHBDD1 Antibody - BSA Free [NBP1-76248] - WB Validation in A549 Cells.Loading: 15 ug of cell lysate Antibodies: RHBDD1, 1 ug/ml, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min

Applications for RHBDD1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RHBDD1

The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.

Alternate Names

DKFZp547E052, EC 3.4.21, EC 3.4.21.-, MGC117258, rhomboid domain containing 1, rhomboid domain-containing protein 1

Gene Symbol

RHBDD1

UniProt

AAI01265 (Human)

Additional RHBDD1 Products

Product Documents for RHBDD1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RHBDD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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