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RHBDD2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47305

Novus Biologicals, part of Bio-Techne
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NBP2-47305

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ERVALKLDQTFPFSLMRRISVFKYVSGSSAERRAAQSRKLNPVPGSYPTQSCHPHLSPSHPVSQTQHASGQKLA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RHBDD2 Antibody

Western Blot: RHBDD2 Antibody [NBP2-47305]

Western Blot: RHBDD2 Antibody [NBP2-47305]

Western Blot: RHBDD2 Antibody [NBP2-47305] - Analysis in control (vector only transfected HEK293T lysate) and RHBDD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RHBDD2 Antibody [NBP2-47305]

Immunocytochemistry/ Immunofluorescence: RHBDD2 Antibody [NBP2-47305]

Immunocytochemistry/Immunofluorescence: RHBDD2 Antibody [NBP2-47305] - Staining human cell line U-251 MG shows positivity in the golgi apparatus and nucleus but excluded from the nucleoli.
Immunohistochemistry-Paraffin: RHBDD2 Antibody [NBP2-47305]

Immunohistochemistry-Paraffin: RHBDD2 Antibody [NBP2-47305]

Immunohistochemistry-Paraffin: RHBDD2 Antibody [NBP2-47305] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for RHBDD2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RHBDD2

Alternate Names

H_RG122E10.2a, H_RG122E10.2b, rhomboid domain containing 2, rhomboid domain-containing protein 2, rhomboid, veinlet-like 7, veinlet-like 7 (Drosophila)

Gene Symbol

RHBDD2

Additional RHBDD2 Products

Product Documents for RHBDD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RHBDD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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