RHBDD2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47305
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ERVALKLDQTFPFSLMRRISVFKYVSGSSAERRAAQSRKLNPVPGSYPTQSCHPHLSPSHPVSQTQHASGQKLA
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (84%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RHBDD2 Antibody
Western Blot: RHBDD2 Antibody [NBP2-47305]
Western Blot: RHBDD2 Antibody [NBP2-47305] - Analysis in control (vector only transfected HEK293T lysate) and RHBDD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: RHBDD2 Antibody [NBP2-47305]
Immunocytochemistry/Immunofluorescence: RHBDD2 Antibody [NBP2-47305] - Staining human cell line U-251 MG shows positivity in the golgi apparatus and nucleus but excluded from the nucleoli.Immunohistochemistry-Paraffin: RHBDD2 Antibody [NBP2-47305]
Immunohistochemistry-Paraffin: RHBDD2 Antibody [NBP2-47305] - Staining of human skeletal muscle shows no positivity in myocytes as expected.Applications for RHBDD2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RHBDD2
Alternate Names
H_RG122E10.2a, H_RG122E10.2b, rhomboid domain containing 2, rhomboid domain-containing protein 2, rhomboid, veinlet-like 7, veinlet-like 7 (Drosophila)
Gene Symbol
RHBDD2
Additional RHBDD2 Products
Product Documents for RHBDD2 Antibody
Product Specific Notices for RHBDD2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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