RHD Antibody (D7C2) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09042
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Lambda Clone # D7C2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for RHD Antibody (D7C2) - Chimeric - Azide and BSA Free
Immunogen
A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Lambda
Scientific Data Images for RHD Antibody (D7C2) - Chimeric - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: RHD Antibody (D7C2) - Chimeric - Azide and BSA Free [NBP3-09042]](https://resources.bio-techne.com/images/products/RHD-Antibody-D7C2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09042-img0001.jpg "Immunocytochemistry/ Immunofluorescence: RHD Antibody (D7C2) - Chimeric - Azide and BSA Free [NBP3-09042]")
Immunocytochemistry/ Immunofluorescence: RHD Antibody (D7C2) - Chimeric - Azide and BSA Free [NBP3-09042]
Immunocytochemistry/Immunofluorescence: RHD Antibody (D7C2) - Chimeric [NBP3-09042] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of D7C2 (NBP3-09042) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09042, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for RHD Antibody (D7C2) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
FC confirmed the binding of D7C2 to Rh D+ red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2 showed a percentage of 93% lysis (Edelman et al, 1997). D7C2 has been expressed recombinantly using a baculovirus-insect cell expression system (Edelman et al, 1997).
FC confirmed the binding of D7C2 to Rh D+ red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2 showed a percentage of 93% lysis (Edelman et al, 1997). D7C2 has been expressed recombinantly using a baculovirus-insect cell expression system (Edelman et al, 1997).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RHD
Alternate Names
blood group Rh(D) polypeptide, CD240D, DIIIc, RH, Rh blood group, D antigen, RH30, Rh4, RHCED, RhDCw, RHDel, RHDVA(TT), RhII, RhK562-II, RhPI, RHPII, RHXIII
Gene Symbol
RHD
Additional RHD Products
Product Documents for RHD Antibody (D7C2) - Chimeric - Azide and BSA Free
Product Specific Notices for RHD Antibody (D7C2) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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