RhoD Antibody (OTI2F7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73889
![Western Blot: RhoD Antibody (OTI2F7)Azide and BSA Free [NBP2-73889]](https://resources.bio-techne.com/images/products/RhoD-Antibody-OTI2F7-Azide-and-BSA-Free-Western-Blot-NBP2-73889-img0001.jpg "Western Blot: RhoD Antibody (OTI2F7)Azide and BSA Free [NBP2-73889]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI2F7
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for RhoD Antibody (OTI2F7) - Azide and BSA Free
Immunogen
Full length human recombinant protein of human RHOD(NP_055393) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
23.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RhoD Antibody (OTI2F7) - Azide and BSA Free
Western Blot: RhoD Antibody (OTI2F7)Azide and BSA Free [NBP2-73889]
Western Blot: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoD.![Immunocytochemistry/ Immunofluorescence: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]](https://resources.bio-techne.com/images/products/RhoD-Antibody-OTI2F7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73889-img0003.jpg "Immunocytochemistry/ Immunofluorescence: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]")
Immunocytochemistry/ Immunofluorescence: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]
Immunocytochemistry/Immunofluorescence: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RhoD.![Flow Cytometry: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]](https://resources.bio-techne.com/images/products/RhoD-Antibody-OTI2F7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73889-img0002.jpg "Flow Cytometry: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]")
Flow Cytometry: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]
Flow Cytometry: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RhoD antibody, and then analyzed by flow cytometry.Applications for RhoD Antibody (OTI2F7) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RhoD
Alternate Names
ARHDRHOM, ras homolog D, ras homolog gene family, member A, ras homolog gene family, member D, Rho, RhoD, RhoHP1, rho-related GTP-binding protein RhoD, Rho-related protein HP1
Gene Symbol
RHOD
Additional RhoD Products
Product Documents for RhoD Antibody (OTI2F7) - Azide and BSA Free
Product Specific Notices for RhoD Antibody (OTI2F7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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