RhoD Antibody (OTI2F7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73889

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2F7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RhoD Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RHOD(NP_055393) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

23.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoD Antibody (OTI2F7) - Azide and BSA Free

Western Blot: RhoD Antibody (OTI2F7)Azide and BSA Free [NBP2-73889]

Western Blot: RhoD Antibody (OTI2F7)Azide and BSA Free [NBP2-73889]

Western Blot: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoD.
Immunocytochemistry/ Immunofluorescence: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]

Immunocytochemistry/ Immunofluorescence: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]

Immunocytochemistry/Immunofluorescence: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RhoD.
Flow Cytometry: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]

Flow Cytometry: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889]

Flow Cytometry: RhoD Antibody (OTI2F7) - Azide and BSA Free [NBP2-73889] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RhoD antibody, and then analyzed by flow cytometry.

Applications for RhoD Antibody (OTI2F7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RhoD

Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. The protein encoded by this gene binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. (provided by RefSeq)

Alternate Names

ARHDRHOM, ras homolog D, ras homolog gene family, member A, ras homolog gene family, member D, Rho, RhoD, RhoHP1, rho-related GTP-binding protein RhoD, Rho-related protein HP1

Gene Symbol

RHOD

Additional RhoD Products

Product Documents for RhoD Antibody (OTI2F7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RhoD Antibody (OTI2F7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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