RhoG Antibody (PSH02-70)

Western Blot: RhoG Antibody (PSH02-70) [NBP3-32902]

Western Blot: RhoG Antibody (PSH02-70) [NBP3-32902] - Western blot analysis of RhoG on different lysates with Rabbit anti-RhoG antibody (NBP3-32902) at 1/2,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: MDA-MB-231 cell lysate
Lane 3: K-562 cell lysate
Lane 4: Hela cell lysate
Lane 5: MCF7 cell lysate
Lane 6: PANC-1 cell lysate
Lane 7: COS-1 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 21 kDa
Observed band size: 21 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32902) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: RhoG Antibody (PSH02-70) [NBP3-32902]

Flow Cytometry: RhoG Antibody (PSH02-70) [NBP3-32902] - Flow cytometric analysis of HeLa cells labeling RhoG.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32902, 1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).