RhoJ Antibody (1E4)

Novus Biologicals, part of Bio-Techne | Catalog # H00057381-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 1E4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RhoJ Antibodies »

Product Specifications

Immunogen

RHOJ (AAH62575, 1 a.a. ~ 214 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNCKEGTDSSCGCRGNDEKKMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHLLGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKRCSEGHSCCSII

Reactivity Notes

Other species not tested.

Specificity

RHOJ - ras homolog gene family, member J

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Scientific Data Images for RhoJ Antibody (1E4)

Western Blot: RhoJ Antibody (1E4) [H00057381-M01]

Western Blot: RhoJ Antibody (1E4) [H00057381-M01]

Western Blot: RhoJ Antibody (1E4) [H00057381-M01] - RHOJ expression in HepG2 ( Cat # L019V1 ).
Immunocytochemistry/ Immunofluorescence: RhoJ Antibody (1E4) [H00057381-M01]

Immunocytochemistry/ Immunofluorescence: RhoJ Antibody (1E4) [H00057381-M01]

Immunocytochemistry/Immunofluorescence: RhoJ Antibody (1E4) [H00057381-M01] - Analysis of monoclonal antibody to RHOJ on HeLa cell. Antibody concentration 60 ug/ml.
ELISA: RhoJ Antibody (1E4) [H00057381-M01]

ELISA: RhoJ Antibody (1E4) [H00057381-M01]

ELISA: RhoJ Antibody (1E4) [H00057381-M01] - Detection limit for recombinant GST tagged RHOJ is approximately 1ng/ml as a capture antibody.

Applications for RhoJ Antibody (1E4)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RhoJ

ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.[supplied by OMIM]

Alternate Names

ARHJTC10B, FLJ14445, ras homolog gene family, member J, RASL7B, Ras-like protein family member 7B, RAS-like, family 7, member B, RHOI, rho-related GTP-binding protein RhoJ, Tc10-like GTP-binding protein, TC10-like Rho GTPase, TCLMGC34777

Entrez Gene IDs

57381 (Human)

Gene Symbol

RHOJ

OMIM

607653 (Human)

UniProt

AAH62575 (Human)

Additional RhoJ Products

Product Documents for RhoJ Antibody (1E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RhoJ Antibody (1E4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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