RhoJ Antibody (1E4)
Novus Biologicals, part of Bio-Techne | Catalog # H00057381-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Lambda Clone # 1E4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RHOJ (AAH62575, 1 a.a. ~ 214 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNCKEGTDSSCGCRGNDEKKMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHLLGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKRCSEGHSCCSII
Reactivity Notes
Other species not tested.
Specificity
RHOJ - ras homolog gene family, member J
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 Lambda
Scientific Data Images for RhoJ Antibody (1E4)
![Western Blot: RhoJ Antibody (1E4) [H00057381-M01]](https://resources.bio-techne.com/images/products/RhoJ-Antibody-1E4-Western-Blot-H00057381-M01-img0001.jpg "Western Blot: RhoJ Antibody (1E4) [H00057381-M01]")
Western Blot: RhoJ Antibody (1E4) [H00057381-M01]
Western Blot: RhoJ Antibody (1E4) [H00057381-M01] - RHOJ expression in HepG2 ( Cat # L019V1 ).![Immunocytochemistry/ Immunofluorescence: RhoJ Antibody (1E4) [H00057381-M01]](https://resources.bio-techne.com/images/products/RhoJ-Antibody-1E4-Immunocytochemistry-Immunofluorescence-H00057381-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: RhoJ Antibody (1E4) [H00057381-M01]")
Immunocytochemistry/ Immunofluorescence: RhoJ Antibody (1E4) [H00057381-M01]
Immunocytochemistry/Immunofluorescence: RhoJ Antibody (1E4) [H00057381-M01] - Analysis of monoclonal antibody to RHOJ on HeLa cell. Antibody concentration 60 ug/ml.![ELISA: RhoJ Antibody (1E4) [H00057381-M01]](https://resources.bio-techne.com/images/products/RhoJ-Antibody-1E4-Sandwich-ELISA-H00057381-M01-img0006.jpg "ELISA: RhoJ Antibody (1E4) [H00057381-M01]")
ELISA: RhoJ Antibody (1E4) [H00057381-M01]
ELISA: RhoJ Antibody (1E4) [H00057381-M01] - Detection limit for recombinant GST tagged RHOJ is approximately 1ng/ml as a capture antibody.Applications for RhoJ Antibody (1E4)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RhoJ
Alternate Names
ARHJTC10B, FLJ14445, ras homolog gene family, member J, RASL7B, Ras-like protein family member 7B, RAS-like, family 7, member B, RHOI, rho-related GTP-binding protein RhoJ, Tc10-like GTP-binding protein, TC10-like Rho GTPase, TCLMGC34777
Entrez Gene IDs
57381 (Human)
Gene Symbol
RHOJ
OMIM
607653 (Human)
UniProt
Additional RhoJ Products
Product Documents for RhoJ Antibody (1E4)
Product Specific Notices for RhoJ Antibody (1E4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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