Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1D7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RhoJ Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoJ Antibody (OTI1D7) - Azide and BSA Free

Western Blot: RhoJ Antibody (OTI1D7)Azide and BSA Free [NBP2-73892]

Western Blot: RhoJ Antibody (OTI1D7)Azide and BSA Free [NBP2-73892]

Western Blot: RhoJ Antibody (OTI1D7) - Azide and BSA Free [NBP2-73892] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RHOJ.
Immunocytochemistry/ Immunofluorescence: RhoJ Antibody (OTI1D7) - Azide and BSA Free [NBP2-73892]

Immunocytochemistry/ Immunofluorescence: RhoJ Antibody (OTI1D7) - Azide and BSA Free [NBP2-73892]

Immunocytochemistry/Immunofluorescence: RhoJ Antibody (OTI1D7) - Azide and BSA Free [NBP2-73892] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RHOJ.
Immunohistochemistry: RhoJ Antibody (OTI1D7) - Azide and BSA Free [NBP2-73892]

Immunohistochemistry: RhoJ Antibody (OTI1D7) - Azide and BSA Free [NBP2-73892]

Immunohistochemistry: RhoJ Antibody (OTI1D7) - Azide and BSA Free [NBP2-73892] - Analysis of Human prostate tissue.
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Applications for RhoJ Antibody (OTI1D7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RhoJ

ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.[supplied by OMIM]

Alternate Names

ARHJTC10B, FLJ14445, ras homolog gene family, member J, RASL7B, Ras-like protein family member 7B, RAS-like, family 7, member B, RHOI, rho-related GTP-binding protein RhoJ, Tc10-like GTP-binding protein, TC10-like Rho GTPase, TCLMGC34777

Gene Symbol

RHOJ

Additional RhoJ Products

Product Documents for RhoJ Antibody (OTI1D7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RhoJ Antibody (OTI1D7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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