RhoJ Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89012

Novus Biologicals, part of Bio-Techne
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NBP1-89012
NBP1-89012-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RhoJ Antibodies »

Product Summary for RhoJ Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKRCSEG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RhoJ Antibody

Western Blot: RhoJ Antibody [NBP1-89012]

Western Blot: RhoJ Antibody [NBP1-89012]

Western Blot: RhoJ Antibody [NBP1-89012] - Analysis in control (vector only transfected HEK293T lysate) and RHOJ over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: RhoJ Antibody [NBP1-89012]

Immunohistochemistry-Paraffin: RhoJ Antibody [NBP1-89012]

Immunohistochemistry-Paraffin: RhoJ Antibody [NBP1-89012] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Applications for RhoJ Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RhoJ

ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.[supplied by OMIM]

Alternate Names

ARHJTC10B, FLJ14445, ras homolog gene family, member J, RASL7B, Ras-like protein family member 7B, RAS-like, family 7, member B, RHOI, rho-related GTP-binding protein RhoJ, Tc10-like GTP-binding protein, TC10-like Rho GTPase, TCLMGC34777

Gene Symbol

RHOJ

Additional RhoJ Products

Product Documents for RhoJ Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RhoJ Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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