Ribophorin I Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55523
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Rat
Cited:
Rat
Predicted:
Mouse (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQ
Reactivity Notes
Use in Rat reported in scientific literature (PMID:34923175) .
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ribophorin I Antibody
Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523]
Immunohistochemistry-Paraffin: Ribophorin I Antibody [NBP2-55523] - Staining of human cerebral cortex, colon, liver and tonsil using Anti-RPN1 antibody NBP2-55523 (A) shows similar protein distribution across tissues to independent antibody NBP1-82467 (B).Western Blot: Ribophorin I Antibody [NBP2-55523]
Western Blot: Ribophorin I Antibody [NBP2-55523] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.Immunocytochemistry/ Immunofluorescence: Ribophorin I Antibody [NBP2-55523]
Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody [NBP2-55523] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.Applications for Ribophorin I Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC Retrieval method: HIER pH6.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ribophorin I
Alternate Names
DKFZp686B16177, dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, EC 2.4.1.119, oligosaccharyltransferase 1 homolog, OST1, ribophorin IRBPH1, ribophorin-1, RPN-I
Gene Symbol
RPN1
Additional Ribophorin I Products
Product Documents for Ribophorin I Antibody
Product Specific Notices for Ribophorin I Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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