Ribosomal protein S27 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1103

Novus Biologicals, part of Bio-Techne
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NB100-1103
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Ribosomal protein S27 Antibodies »

Product Summary for Ribosomal protein S27 Antibody

Immunogen

Peptide with sequence PLAKDLLHPSPEE-C corresponding to N-Terminus according to NP_001021.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Ribosomal protein S27 Antibody

Western Blot: Ribosomal protein S27 Antibody [NB100-1103]

Western Blot: Ribosomal protein S27 Antibody [NB100-1103]

Western Blot: Ribosomal protein S27 Antibody [NB100-1103] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Ribosomal protein S27 Antibody [NB100-1103]

Immunohistochemistry-Paraffin: Ribosomal protein S27 Antibody [NB100-1103]

Immunohistochemistry-Paraffin: Ribosomal protein S27 Antibody [NB100-1103] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Ribosomal protein S27 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 12 kDa band observed in lysates of human T-cell line Jurkat (calculated MW of 9.5 kDa band according to NP_001021.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ribosomal protein S27

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Alternate Names

Metallopan-stimulin 1, MPS140S ribosomal protein S27, MPS-1metallopanstimulin 1, ribosomal protein S27, ribosomal protein S27 (metallopanstimulin 1)

Entrez Gene IDs

6232 (Human); 57294 (Mouse); 94266 (Rat)

Gene Symbol

RPS27

UniProt

NP_001021. (Human)

Additional Ribosomal protein S27 Products

Product Documents for Ribosomal protein S27 Antibody

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Product Specific Notices for Ribosomal protein S27 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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