Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G3

Concentration

0.83 mg/ml

View all RIC8A Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIC8A Antibody (OTI3G3)

Western Blot: RIC8A Antibody (OTI3G3) [NBP2-02433]

Western Blot: RIC8A Antibody (OTI3G3) [NBP2-02433]

Western Blot: RIC8 Antibody (3G3) [NBP2-02433] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8.
Immunocytochemistry/Immunofluorescence: RIC8 Antibody (3G3) [NBP2-02433] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8.
Immunohistochemistry-Paraffin: RIC8A Antibody (OTI3G3) [NBP2-02433]

Immunohistochemistry-Paraffin: RIC8A Antibody (OTI3G3) [NBP2-02433]

Immunohistochemistry-Paraffin: RIC8 Antibody (3G3) [NBP2-02433] - Staining of paraffin-embedded Kidney tissue using anti-RIC8 mouse monoclonal antibody.
View 1 more images

Applications for RIC8A Antibody (OTI3G3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.83 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RIC8A

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation

Alternate Names

RIC8, RIC8A resistance to inhibitors of cholinesterase 8 homolog A (C. elegans), synembryn, synembryn-A

Entrez Gene IDs

60626 (Human)

Gene Symbol

RIC8A

Additional RIC8A Products

Product Documents for RIC8A Antibody (OTI3G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RIC8A Antibody (OTI3G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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