Ring finger protein 214 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87994
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (93%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VTRSLKAGCHTKQLASRNCSEEKSPQTSILKEGNRDTSLDFRPVVSPANGVEGVRVDQDDDQDSSSLKLSQNIAVQTDFKTADSEVNTDQDIEKNLD
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ring finger protein 214 Antibody
![Western Blot: Ring finger protein 214 Antibody [NBP1-87994]](https://resources.bio-techne.com/images/products/Ring-finger-protein-214-Antibody-Western-Blot-NBP1-87994-img0006.jpg "Western Blot: Ring finger protein 214 Antibody [NBP1-87994]")
Western Blot: Ring finger protein 214 Antibody [NBP1-87994]
Western Blot: Ring finger protein 214 Antibody [NBP1-87994] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4, Lane 3: Human cell line U-251MG sp![Immunocytochemistry/ Immunofluorescence: Ring finger protein 214 Antibody [NBP1-87994]](https://resources.bio-techne.com/images/products/Ring-finger-protein-214-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87994-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Ring finger protein 214 Antibody [NBP1-87994]")
Immunocytochemistry/ Immunofluorescence: Ring finger protein 214 Antibody [NBP1-87994]
Immunocytochemistry/Immunofluorescence: Ring finger protein 214 Antibody [NBP1-87994] - Staining of human cell line A-431 shows positivity in cytoplasm and golgi apparatus.![Immunohistochemistry-Paraffin: Ring finger protein 214 Antibody [NBP1-87994]](https://resources.bio-techne.com/images/products/Ring-finger-protein-214-Antibody-Immunohistochemistry-Paraffin-NBP1-87994-img0002.jpg "Immunohistochemistry-Paraffin: Ring finger protein 214 Antibody [NBP1-87994]")
Immunohistochemistry-Paraffin: Ring finger protein 214 Antibody [NBP1-87994]
Immunohistochemistry-Paraffin: Ring finger protein 214 Antibody [NBP1-87994] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.Applications for Ring finger protein 214 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ring finger protein 214
Alternate Names
DKFZp547C195, ring finger protein 214
Gene Symbol
RNF214
Additional Ring finger protein 214 Products
Product Documents for Ring finger protein 214 Antibody
Product Specific Notices for Ring finger protein 214 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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