RING1 Antibody (1F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006015-M10

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RING1 Antibodies »

Product Specifications

Immunogen

RING1 (NP_002922, 81 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPIPGSDQTTTMS

Specificity

RING1 - ring finger protein 1 (1F4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RING1 Antibody (1F4)

Western Blot: RING1 Antibody (1F4) [H00006015-M10]

Western Blot: RING1 Antibody (1F4) [H00006015-M10]

Western Blot: RING1 Antibody (1F4) [H00006015-M10] - Analysis of RING1 expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: RING1 Antibody (1F4) [H00006015-M10]

Immunocytochemistry/ Immunofluorescence: RING1 Antibody (1F4) [H00006015-M10]

Immunocytochemistry/Immunofluorescence: RING1 Antibody (1F4) [H00006015-M10] - Analysis of monoclonal antibody to RING1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: RING1 Antibody (1F4) [H00006015-M10] - Detection limit for recombinant GST tagged RING1 is 3 ng/ml as a capture antibody.

Applications for RING1 Antibody (1F4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RING1

This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq]

Long Name

E3 ubiquitin-protein ligase RING1

Alternate Names

RNF1

Entrez Gene IDs

6015 (Human)

Gene Symbol

RING1

UniProt

NP_002922 (Human)

Additional RING1 Products

Product Documents for RING1 Antibody (1F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RING1 Antibody (1F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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