Key Product Details

Species Reactivity

Human, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RIPK1/RIP1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 133-422 of human RIPK1(NP_003795) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

75.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RIPK1/RIP1 Antibody (OTI2D6) - Azide and BSA Free

Western Blot: RIPK1/RIP1 Antibody (OTI2D6)Azide and BSA Free [NBP2-73911]

Western Blot: RIPK1/RIP1 Antibody (OTI2D6)Azide and BSA Free [NBP2-73911]

Western Blot: RIPK1/RIP1 Antibody (OTI2D6) - Azide and BSA Free [NBP2-73911] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK1.
Immunohistochemistry: RIPK1/RIP1 Antibody (OTI2D6) - Azide and BSA Free [NBP2-73911]

Immunohistochemistry: RIPK1/RIP1 Antibody (OTI2D6) - Azide and BSA Free [NBP2-73911]

Immunohistochemistry: RIPK1/RIP1 Antibody (OTI2D6) - Azide and BSA Free [NBP2-73911] - Analysis of Human Kidney tissue.
Western Blot: RIPK1/RIP1 Antibody (OTI2D6)Azide and BSA Free [NBP2-73911]

Western Blot: RIPK1/RIP1 Antibody (OTI2D6)Azide and BSA Free [NBP2-73911]

Western Blot: RIPK1/RIP1 Antibody (OTI2D6) - Azide and BSA Free [NBP2-73911] - Analysis of extracts (10ug) from 2 different cell lines COS7: monkey; MDCK: canine.
View 6 more images

Applications for RIPK1/RIP1 Antibody (OTI2D6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RIPK1/RIP1

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1

Alternate Names

RIP, RIP1

Gene Symbol

RIPK1

Additional RIPK1/RIP1 Products

Product Documents for RIPK1/RIP1 Antibody (OTI2D6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RIPK1/RIP1 Antibody (OTI2D6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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