Rit1 Antibody (14G7)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-13093
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 14G7
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full length protein (Human).
Reactivity Notes
Cross-reacts with Human and Mouse. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Cell Membrane
Specificity
This does not cross-react with Rit2 protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
~36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications for Rit1 Antibody (14G7)
Application
Recommended Usage
Western Blot
0.5-1 ug/ml
Application Notes
WB: Use HEK293 cells transfected with full-length human Rit1 protein. Untransfected HEK293 cells are used as a negative control to determine the extent of non-specific binding. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa).
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
20mM Sodium Phosphate , 150mM NaCl, 50% Glycerol (pH 7.5)
Preservative
3mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Rit1
Long Name
Ras-like without CAAX 1
Alternate Names
RIBB, ROC1
Gene Symbol
RIT1
Additional Rit1 Products
Product Documents for Rit1 Antibody (14G7)
Product Specific Notices for Rit1 Antibody (14G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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