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RNA polymerase I termination factor Antibody (2F4D8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27122

Novus Biologicals, part of Bio-Techne
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NBP3-27122-0.025ml
NBP3-27122-0.1ml

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F4D8

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human RNA polymerase I termination factor (AA: 1-150) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNA polymerase I termination factor Antibody (2F4D8)

Immunohistochemistry: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

Immunohistochemistry: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Analysis of paraffin-embedded rectum cancer tissues using RNA polymerase I termination factor mouse mAb with DAB staining.

Western Blot: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

Western Blot: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Analysis using RNA polymerase I termination factor mAb against human RNA polymerase I termination factor (AA: 1-150) recombinant protein. (Expected MW is 43.5 kDa)

ELISA: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] -

ELISA: RNA polymerase I termination factor Antibody (2F4D8) [NBP3-27122] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Applications for RNA polymerase I termination factor Antibody (2F4D8)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNA polymerase I termination factor

The TTF1 gene encodes a 905 amino acid long, 103 kDA transcription termination factor 1 protein, also known as a RNA polymerase I termination factor, which functions in terminating ribosomal gene transcription. Additionally, it regulates fork arrest replication and RNA polymerase I transcription on chromatin. Through monitoring the activation as well as the silencing of rDNA transcription, TTF1 acts in rDNA regulation. TTF1 functions in RNA polymerase I transcription as well as mitochondrial transcription and gene expression. It is known to interact with genes PTRF, NFATC3, BAZ2A, SMAD1, and SMAD7. TTF1 has been researched regarding its role in lung cancer, small cell carcinoma, sex cord-gonadal stromal tumor, thyroiditis, blastoma, adenocarcinoma, desmoplastic small round cell tumors, and hirschsprung's disease.

Alternate Names

RNA polymerase I termination factor, transcription termination factor 1, Transcription termination factor I, transcription termination factor, RNA polymerase I, TTF-1, TTF-I

Gene Symbol

TTF1

Additional RNA polymerase I termination factor Products

Product Documents for RNA polymerase I termination factor Antibody (2F4D8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNA polymerase I termination factor Antibody (2F4D8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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