RNASEH2A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76981

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all RNASEH2A Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A. The immunogen is located within amino acids 190 - 240 of RNAse H2A. Amino Acid Squence: LDTDYGSGYPNDPKTKA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNASEH2A Antibody - BSA Free

Western Blot: RNASEH2A AntibodyBSA Free [NBP1-76981]

Western Blot: RNASEH2A AntibodyBSA Free [NBP1-76981]

Western Blot: RNASEH2A Antibody [NBP1-76981] - HeLa cell lysate with RNAse H2A antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: RNASEH2A Antibody - BSA Free [NBP1-76981]

Immunocytochemistry/ Immunofluorescence: RNASEH2A Antibody - BSA Free [NBP1-76981]

Immunocytochemistry/Immunofluorescence: RNASEH2A Antibody [NBP1-76981] - HeLa cells with RNASEH2A antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: RNASEH2A Antibody - BSA Free [NBP1-76981]

Immunocytochemistry/ Immunofluorescence: RNASEH2A Antibody - BSA Free [NBP1-76981]

Immunocytochemistry/Immunofluorescence: RNASEH2A Antibody [NBP1-76981] - Hela cells with RNAse H2A antibody at 5 ug/mL.
View 4 more images

Applications for RNASEH2A Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2-4 ug/ml

Western Blot

1 ug/ml
Application Notes
IP reactivity reported in (PMID: 26106861).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNASEH2A

Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.

Alternate Names

AGS4RNase H2 subunit A, Aicardi-Goutieres syndrome 4, Aicardi-Goutieres syndrome 4 protein, JUNB, ribonuclease H2 subunit A, ribonuclease H2, large subunit, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, ribonuclease HI, large subunit, RNase H(35), RNASEHIEC 3.1.26.4, RNHIARNase HI large subunit, RNHL

Gene Symbol

RNASEH2A

UniProt

O75792 (Human)

Additional RNASEH2A Products

Product Documents for RNASEH2A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RNASEH2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.