RNF111 Antibody (1C4)
Novus Biologicals, part of Bio-Techne | Catalog # H00054778-M05
![ELISA: RNF111 Antibody (1C4) [H00054778-M05]](https://resources.bio-techne.com/images/products/RNF111-Antibody-1C4-ELISA-H00054778-M05-img0006.jpg "ELISA: RNF111 Antibody (1C4) [H00054778-M05]")
Key Product Details
Species Reactivity
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1C4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RNF111 (NP_060080, 1 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSQWTPEYNELYTLKVDMKSEIPSDAPKTQESLKGILLHPEPIGAAKSFPAGVEMINSKVGNEFSHLCDDSQKQEKEMNGNQQEQEKSLVVRKKRKSQQAGPSYVQNC
Specificity
RNF111 - ring finger protein 111
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for RNF111 Antibody (1C4)
![Western Blot: RNF111 Antibody (1C4) [H00054778-M05]](https://resources.bio-techne.com/images/products/RNF111-Antibody-1C4-Western-Blot-H00054778-M05-img0005.jpg "Western Blot: RNF111 Antibody (1C4) [H00054778-M05]")
Western Blot: RNF111 Antibody (1C4) [H00054778-M05]
Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of RNF111 expression in transfected 293T cell line by RNF111 monoclonal antibody (M05), clone 1C4. Lane 1: RNF111 transfected lysatE (107.8 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05]](https://resources.bio-techne.com/images/products/RNF111-Antibody-1C4-Immunocytochemistry-Immunofluorescence-H00054778-M05-img0003.jpg "Immunocytochemistry/ Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05]")
Immunocytochemistry/ Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05]
Immunocytochemistry/Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of monoclonal antibody to RNF111 on HeLa cell. Antibody concentration 10 ug/ml![Western Blot: RNF111 Antibody (1C4) [H00054778-M05]](https://resources.bio-techne.com/images/products/RNF111-Antibody-1C4-Western-Blot-H00054778-M05-img0002.jpg "Western Blot: RNF111 Antibody (1C4) [H00054778-M05]")
Western Blot: RNF111 Antibody (1C4) [H00054778-M05]
Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Western blot analysis of RNF111 over-expressed 293 cell line, cotransfected with RNF111 Validated Chimera RNAi or non-transfected control. Blot probed with H00054778-M05. GAPDH (36.1 kDa) used as loading control.Applications for RNF111 Antibody (1C4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validaton and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNF111
Alternate Names
ARK, Arkadia, DKFZp313E0731, DKFZp761D081, E3 ubiquitin-protein ligase Arkadia, EC 6.3.2, EC 6.3.2.-, FLJ38008, ring finger protein 111DKFZp686H1966
Entrez Gene IDs
54778 (Human)
Gene Symbol
RNF111
OMIM
605840 (Human)
UniProt
Additional RNF111 Products
Product Documents for RNF111 Antibody (1C4)
Product Specific Notices for RNF111 Antibody (1C4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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