RNF12 Antibody (1G10)
Novus Biologicals, part of Bio-Techne | Catalog # H00051132-M01
![Immunoprecipitation: RNF12 Antibody (1G10) [H00051132-M01]](https://resources.bio-techne.com/images/products/RNF12-Antibody-1G10-Immunoprecipitation-H00051132-M01-img0007.jpg "Immunoprecipitation: RNF12 Antibody (1G10) [H00051132-M01]")
![ELISA: RNF12 Antibody (1G10) [H00051132-M01]](https://resources.bio-techne.com/images/products/RNF12-Antibody-1G10-ELISA-H00051132-M01-img0011.jpg "ELISA: RNF12 Antibody (1G10) [H00051132-M01]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1G10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RNF12 (NP_057204, 1 a.a. ~ 83 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDEN
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 27788132).
Localization
Cytoplasmic and Nuclear
Specificity
RNF12 - ring finger protein 12
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for RNF12 Antibody (1G10)
![Western Blot: RNF12 Antibody (1G10) [H00051132-M01]](https://resources.bio-techne.com/images/products/RNF12-Antibody-1G10-Western-Blot-H00051132-M01-img0009.jpg "Western Blot: RNF12 Antibody (1G10) [H00051132-M01]")
Western Blot: RNF12 Antibody (1G10) [H00051132-M01]
Western Blot: RNF12 Antibody (1G10) [H00051132-M01] - RNF12 monoclonal antibody (M01), clone 1G10. Analysis of RNF12 expression in Hela S3 NE.![Immunocytochemistry/ Immunofluorescence: RNF12 Antibody (1G10) [H00051132-M01]](https://resources.bio-techne.com/images/products/RNF12-Antibody-1G10-Immunocytochemistry-Immunofluorescence-H00051132-M01-img0006.jpg "Immunocytochemistry/ Immunofluorescence: RNF12 Antibody (1G10) [H00051132-M01]")
Immunocytochemistry/ Immunofluorescence: RNF12 Antibody (1G10) [H00051132-M01]
Immunocytochemistry/Immunofluorescence: RNF12 Antibody (1G10) [H00051132-M01] - Analysis of monoclonal antibody to RNF12 on HeLa cell. Antibody concentration 30 ug/ml.![Western Blot: RNF12 Antibody (1G10) [H00051132-M01]](https://resources.bio-techne.com/images/products/RNF12-Antibody-1G10-Western-Blot-H00051132-M01-img0010.jpg "Western Blot: RNF12 Antibody (1G10) [H00051132-M01]")
Western Blot: RNF12 Antibody (1G10) [H00051132-M01]
Western Blot: RNF12 Antibody (1G10) [H00051132-M01] - Analysis of RNF12 expression in transfected 293T cell line by RNF12 monoclonal antibody (M01), clone 1G10.Lane 1: RNF12 transfected lysate (Predicted MW: 68.5 KDa).Lane 2: Non-transfected lysate.Applications for RNF12 Antibody (1G10)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNF12
Alternate Names
DKFZp686N06224, E3 ubiquitin-protein ligase RLIM, E3 ubiquitin-protein ligase RNF12, EC 6.3.2, EC 6.3.2.-, FLJ25923, FLJ41093, LIM domain-interacting RING finger protein, MGC15161, NY-REN-43, Renal carcinoma antigen NY-REN-43, RING finger LIM domain-binding protein, RING finger protein 12FLJ42887, ring finger protein, LIM domain interacting, ring zinc finger LIM domain binding protein, ring zinc finger protein NY-REN-43antigen, R-LIM, RNF12FLJ45986
Entrez Gene IDs
51132 (Human)
Gene Symbol
RLIM
OMIM
300379 (Human)
UniProt
Additional RNF12 Products
Product Documents for RNF12 Antibody (1G10)
Product Specific Notices for RNF12 Antibody (1G10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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