RNF168 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76324
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human RNF168. The immunogen is located within amino acids 440 - 490 of RNF168. Amino Acid Squence: KEVDKEQMVPNRQKGSPD
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%), Bovine (82%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNF168 Antibody - BSA Free
Western Blot: RNF168 AntibodyBSA Free [NBP1-76324]
Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] - RNF168 Antibody [NBP1-76324] - Western blot analysis showing the expression of BMI1, RING1A, RING1B, EZH2, gamma-H2AX, Ub-H2A, p-CHK2, p-ATM, RNF8, RNF168, MEL18, p53BP, BRCA1 proteins in MDAMB-231cells transfected with miR-15a, miR-16 or both miR-15a/16 under etoposide-induced DNA damage conditions. Actin served as a gel loading control. Blots were cropped to enhance the representation. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-02800-2), licensed under a CC-BY license.Western Blot: RNF168 AntibodyBSA Free [NBP1-76324]
Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] - RNF168 Antibody [NBP1-76324] - Human brain tissue lysate with RNF168 antibody at 1 ug/mL.Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] -
Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] - Ectopic expression of miR-15a, miR-16 sensitizes breast cancer cells to DNA damage through BMI1 dependent ubiquitination pathway. Western blot analysis showing the expression of BMI1, RING1A, RING1B, EZH2, gamma-H2AX, Ub-H2A, p-CHK2, p-ATM, RNF8, RNF168, MEL18, p53BP, BRCA1 proteins in MDAMB-231 cells transfected with miR-15a, miR-16 or both miR-15a/16 under etoposide-induced DNA damage conditions. Actin served as a gel loading control. Blots were cropped to enhance the representation (A,B). Immunofluorescence data showing accumulation of BMI1, ϒ-H2AX (C) RING1B (D) & Ub-H2A (E) p53BP (F) in MDAMB-231 cells transfected with miR-15a miR-16 or both miR-15a/16 under etoposide- treated conditions. Bar indicates 200 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28655885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for RNF168 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNF168
Long Name
Ring Finger Protein 168
Alternate Names
E3 ubiquitin-protein ligase RNF168, EC 6.3.2, EC 6.3.2.-, FLJ39749, ring finger protein 168FLJ35794
Gene Symbol
RNF168
UniProt
Additional RNF168 Products
Product Documents for RNF168 Antibody - BSA Free
Product Specific Notices for RNF168 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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