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RNF182 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82707

Novus Biologicals, part of Bio-Techne
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NBP1-82707

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunohistochemistry-Paraffin, Knockdown Validated, SDS-Page, Western Blot

Cited:

Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLECCHRVCAKCLYKIIDFGDSPQGVIVCPFCRFETCLPDDEVSSLPDDNNILVNLTCGGKGKKCLPENPTELLLTPKRLASLVSPSHTSSNCLVITIMEVQRESSPSLSSTPVVEFYRPASFDSVTTVSHNWTVWNCTSLL

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35458235).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF182 Antibody

Western Blot: RNF182 Antibody [NBP1-82707]

Western Blot: RNF182 Antibody [NBP1-82707]

Western Blot: RNF182 Antibody [NBP1-82707] - Analysis in control (vector only transfected HEK293T lysate) and RNF182 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: RNF182 Antibody [NBP1-82707]

Immunohistochemistry-Paraffin: RNF182 Antibody [NBP1-82707]

Immunohistochemistry-Paraffin: RNF182 Antibody [NBP1-82707] - Staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.

Applications for RNF182 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
Use in KD reported in scientific literature (PMID:35458235) For IHC-Paraffin, HIER pH 6 retrieval is recommended..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF182

Alternate Names

E3 ubiquitin-protein ligase RNF182, EC 6.3.2.-, FLJ40772, ring finger protein 182MGC33993

Gene Symbol

RNF182

Additional RNF182 Products

Product Documents for RNF182 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNF182 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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