RNF2 Antibody (9Q2W2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16222

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16222-100ul
NBP3-16222-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9Q2W2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RNF2 Antibodies »

Product Summary for RNF2 Antibody (9Q2W2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RNF2 (Q99496). LRPDPNFDALISKIYPSRDEYEAHQERVLARINKHNNQQALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSNASTHSNQEAGPSNKRTKTS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF2 Antibody (9Q2W2)

Western Blot: RNF2 Antibody (9Q2W2) [NBP3-16222]

Western Blot: RNF2 Antibody (9Q2W2) [NBP3-16222]

Western Blot: RNF2 Antibody (9Q2W2) [NBP3-16222] - Western blot analysis of extracts of various cell lines, using RNF2 Rabbit mAb (NBP3-16222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunoprecipitation: RNF2 Antibody (9Q2W2) [NBP3-16222]

Immunoprecipitation: RNF2 Antibody (9Q2W2) [NBP3-16222]

Immunoprecipitation: RNF2 Antibody (9Q2W2) [NBP3-16222] - Immunoprecipitation analysis of 300ug extracts of U-937 cells using 3ug RNF2 antibody (NBP3-16222). Western blot was performed from the immunoprecipitate using RNF2 antibody (NBP3-16222) at a dilition of 1:500.

Applications for RNF2 Antibody (9Q2W2)

Application
Recommended Usage

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF2

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

Long Name

Ring Finger Protein 2

Alternate Names

DING, HIPI3, RING1B, RING2

Gene Symbol

RNF2

Additional RNF2 Products

Product Documents for RNF2 Antibody (9Q2W2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF2 Antibody (9Q2W2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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