RNF20 Antibody (2H12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26341
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2H12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human RNF20 [UniProt Q5VTR2]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RNF20 Antibody (2H12)
Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] -
Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] - Image of RNF20 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] -
Immunohistochemistry: RNF20 Antibody (2H12) [NBP3-26341] - Image of RNF20 Antibody (2H12) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: RNF20 Antibody (2H12) [NBP3-26341] -
Western Blot: RNF20 Antibody (2H12) [NBP3-26341] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate.All lanes: RNF20 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Applications for RNF20 Antibody (2H12)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: RNF20
Alternate Names
BRE1, BRE1 E3 ubiquitin ligase homolog, BRE1-A, BRE1AMGC129667, E3 ubiquitin-protein ligase BRE1A, EC 6.3.2, EC 6.3.2.-, FLJ11189, FLJ20382, hBRE1MGC129668, homolog of S. cerevisiae BRE1, KAIA2779, ring finger protein 20KIAA2779
Gene Symbol
RNF20
Additional RNF20 Products
Product Documents for RNF20 Antibody (2H12)
Product Specific Notices for RNF20 Antibody (2H12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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