RNF212 Antibody (5H3)
Novus Biologicals, part of Bio-Techne | Catalog # H00285498-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5H3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RNF212 (NP_919420, 133 a.a. ~ 232 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IKSSVSTKPHGCLLPPHSSAPDRLESMEVDLSPSPIRKSEIAAGPARISMISPPQDGRMAPCARRVCHFQRFTMFLHRRLSSLAAPPSVQFWKARGTHQL
Reactivity Notes
Human. Other species not tested.
Specificity
LOC285498 - hypothetical protein LOC285498
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for RNF212 Antibody (5H3)
Western Blot: RNF212 Antibody (5H3) [H00285498-M01]
Western Blot: RNF212 Antibody (5H3) [H00285498-M01] - Analysis of RNF212 expression in transfected 293T cell line by RNF212 monoclonal antibody (M01), clone 5H3.Lane 1: RNF212 transfected lysate (Predicted MW: 33.4 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: RNF212 Antibody (5H3) [H00285498-M01]
Immunocytochemistry/Immunofluorescence: RNF212 Antibody (5H3) [H00285498-M01] - Analysis of monoclonal antibody to RNF212 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: RNF212 Antibody (5H3) [H00285498-M01] - Detection limit for recombinant GST tagged RNF212 is 0.1 ng/ml as a capture antibody.
Applications for RNF212 Antibody (5H3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RNF212
Alternate Names
FLJ38841, FLJ42587, hypothetical protein LOC285498, MGC120227, MGC120228, ring finger protein 212, ZHP3
Entrez Gene IDs
285498 (Human)
Gene Symbol
RNF212
UniProt
Additional RNF212 Products
Product Documents for RNF212 Antibody (5H3)
Product Specific Notices for RNF212 Antibody (5H3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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