RNF36 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42073

Novus Biologicals, part of Bio-Techne
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NBP2-42073
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Summary for RNF36 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GDYVEMNDSITHLPSKVVIQDITMELHCPLCNDWFRDPLMLSCGHNFCEACIQDFWRLQAKETFCPECKMLCQYNNCTFNPVLDKLVEKIKKLPLLKGHPQCPEHGENLKLFSKPDGKLICFQCKDARLSVGQSKEFLQISDAV

Reactivity Notes

Mouse (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF36 Antibody

Western Blot: RNF36 Antibody [NBP2-42073]

Western Blot: RNF36 Antibody [NBP2-42073]

Western Blot: RNF36 Antibody [NBP2-42073] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: RNF36 Antibody [NBP2-42073]

Immunohistochemistry-Paraffin: RNF36 Antibody [NBP2-42073]

Immunohistochemistry-Paraffin: RNF36 Antibody [NBP2-42073] - Staining of human prostate shows low expression as expected.
Immunohistochemistry-Paraffin: RNF36 Antibody [NBP2-42073]

Immunohistochemistry-Paraffin: RNF36 Antibody [NBP2-42073]

Immunohistochemistry-Paraffin: RNF36 Antibody [NBP2-42073] - Analysis in human testis and prostate tissues using Anti-TRIM69 antibody. Corresponding TRIM69 RNA-seq data are presented for the same tissues.
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Applications for RNF36 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF36

RNF36 encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Alternate Names

RFP-like domain-containing protein trimless, RING finger protein 36HSD34, RNF36, Trif, TRIMLESS, tripartite motif containing 69, tripartite motif protein 69, tripartite motif-containing 69, tripartite motif-containing protein 69

Gene Symbol

TRIM69

Additional RNF36 Products

Product Documents for RNF36 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF36 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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